Peptide Nucleic Acids (PNA): Custom PNA Oligo Synthesis 🧬

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Peptide Nucleic Acids (PNAs) 🧬 are synthetic singlestranded DNA, or RNA mimics that consist of nucleobases attached to a polyamide backbone. The DNA-like PNAs' sugar-phosphodiester moiety is replaced with an uncharged N-(2-aminoethyl) glycine and the nucleobases are attached via a methyl carbonyl linker. The PNAs can sequence-specifically recognize duplex DNA and have remarkable resistance to DNases and proteinases. PNA provides a powerful tool in research and clinical applications as antisense agents in developing gene-specific therapeutics for various diseases including cancer as novel antibiotics and antivirals. PNAs have been widely used in molecular diagnostics and therapeutics from regulation target gene expression, gene silencing, antisense, antigene agents, molecular probes, biosensors, 8-17 DNAzyme and 10-23 DNAzyme, to fluorescent in-situ hybridization probes (FISH) and single nucleotide polymorphisms (SNPs) detection.

LifeTein provides custom PNA oligos, unlabeled or labeled by fluorescent dyes or other modifications. The PNA oligos can be conjugated to peptides for additional functionality. PNA oligomers can be labeled at 5' and/or 3' end. These labels are available upon request: fluorophores including FAM, FITC, Alexa Fluor dyes, Atto dyes, Cyanine dyes (cy3, cy5, cy7), QSY9, Pyrene, Dylight, quenchers (BHQ, Dabcyl), Acridine, Butyric acid, Alkyne (DBCO or Pentynoic acid), Azide, Biotin, photocleavable biotin, Maleimide, Myristol, Palmitic acid, et al. Contact us if your modification is not on the list.

Probe	Ex (nm)	Em (nm)	MW	Notes
Alexa 488 dye	490	525	699.7
FITC or FAM	495	519	389	pH sensitive
Cyanine Cy2	492	510
Cyanine Cy3	550	570
Alexa 555 dye	555	580	534.5
Rhodamine B	570	590
Abberior Star635	635	655	911	STED and Confocal Imaging
Cyanine Cy5	650	670
Silicon-Rhodamine (SiR)	652	674	472.61	Multicolour Live Cell Imaging

PNA FISH Probes

TelC telomere probe	CCCTAACCCTAACCCTAA; Reverse Complementary Sequence (5' to 3'): TTAGGGTTAGGGTTAGGG
TelG telomere probe	TTAGGGTTAGGGTTAGGG; Reverse Complementary Sequence (5' to 3'): CCCTAACCCTAACCCTAA
Pan-centromere CENPB probe	ATTCGTTGGAAACGGGA; Reverse Complementary Sequence (5' to 3'): TCCCGTTTCCAACGAAT
Human only pan-centromere CENT probe	AAACTAGACAGAAGCATT; Reverse Complementary Sequence (5' to 3'): AATGCTTCTGTCTAGTTT

PNA Applications

• Microarrays and biosensors: PNA microarray combined with PCR could detect genetically modified organisms.
• PCR clamping and artificial restriction enzyme: PNA clamp complementary to wild type sequence hybridizes specifically with wild type and blocks its amplification while allowing amplification of mutant sequence of the imperfect match.
• Imaging probes and FISH of Fluorescent-PNA: The fluorescent dye-conjugated PNA can bind to DNA or RNA quickly, even under low salt.
• Antisense and antigene drugs of Alkyne/azide-PNA: PNA can bind to a complementary sequence of mRNA and change its function. PNA can break up DNA duplex and form PNA/DNA triplex or double duplexes without denaturing the DNA duplex.
• miRNA inhibitors of PNA-CPP: PNA binds complementary RNA more strongly than DNA or RNA does. PNA miRNA inhibitors can be conjugated to cell penetrating peptide without the need for transfection reagents for cell entry.
• Double strand DNA invasion and capture Biotinylated-PNA: Because of its uncharged polyamide backbone, PNA can hybridize to negatively charged DNA or RNA without electrostatic repulsion.

CPP-PNA Examples

CPP	Sequence
Pen	RQIKIWFQNRRMKWKK-PNA
Tat	GRKKRRQRRRPPQ-PNA
47Tat57	GGGGYGRKKRRQRRR-PNA
Cationic	KKKK-PNA
Lys	K-PNA-KKK
Arg	RRRRRRRR-PNA
H region	AAVALLPAVLLALLA-PNA
PTD-4	YARAAARQARA-PNA
Tp-10	AGYLLGKINLKALAALAKKIL-PNA
SSBP(I)	PKKKRKV-PNA
C-myc tag	EQKLISEEDLNA-PNA
Tat-modified	RRRQRRKKR-PNA

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