Peptide Nucleic Acids (PNAs)

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Peptide Nucleic Acids (PNA): Custom PNA Oligo Synthesis 🧬

Peptide Nucleic Acids

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Peptide Nucleic Acids (PNAs) 🧬 are synthetic singlestranded DNA, or RNA mimics that consist of nucleobases attached to a polyamide backbone. The DNA-like PNAs' sugar-phosphodiester moiety is replaced with an uncharged N-(2-aminoethyl) glycine and the nucleobases are attached via a methyl carbonyl linker. The PNAs can sequence-specifically recognize duplex DNA and have remarkable resistance to DNases and proteinases. PNA provides a powerful tool in research and clinical applications as antisense agents in developing gene-specific therapeutics for various diseases including cancer as novel antibiotics and antivirals. PNAs have been widely used in molecular diagnostics and therapeutics from regulation target gene expression, gene silencing, antisense, antigene agents, molecular probes, biosensors, 8-17 DNAzyme and 10-23 DNAzyme, to fluorescent in-situ hybridization probes (FISH) and single nucleotide polymorphisms (SNPs) detection.

LifeTein provides custom PNA oligos, unlabeled or labeled by fluorescent dyes or other modifications. The PNA oligos can be conjugated to peptides for additional functionality. PNA oligomers can be labeled at 5' and/or 3' end. These labels are available upon request: fluorophores including FAM, FITC, Alexa Fluor dyes, Atto dyes, Cyanine dyes (cy3, cy5, cy7), QSY9, Pyrene, Dylight, quenchers (BHQ, Dabcyl), Acridine, Butyric acid, Alkyne (DBCO or Pentynoic acid), Azide, Biotin, photocleavable biotin, Maleimide, Myristol, Palmitic acid, et al. Contact us if your modification is not on the list.

Probe Ex (nm) Em (nm) MW Notes
Alexa 488 dye 490 525 699.7
FITC or FAM 495 519 389 pH sensitive
Cyanine Cy2 492 510
Cyanine Cy3 550 570
Alexa 555 dye 555 580 534.5
Rhodamine B 570 590
Abberior Star635 635 655 911 STED and Confocal Imaging
Cyanine Cy5 650 670
Silicon-Rhodamine (SiR) 652 674 472.61 Multicolour Live Cell Imaging

PNA Applications

  • Microarrays and biosensors: PNA microarray combined with PCR could detect genetically modified organisms.
  • PCR clamping and artificial restriction enzyme: PNA clamp complementary to wild type sequence hybridizes specifically with wild type and blocks its amplification while allowing amplification of mutant sequence of the imperfect match.
  • Imaging probes and FISH of Fluorescent-PNA: The fluorescent dye-conjugated PNA can bind to DNA or RNA quickly, even under low salt.
  • Antisense and antigene drugs of Alkyne/azide-PNA: PNA can bind to a complementary sequence of mRNA and change its function. PNA can break up DNA duplex and form PNA/DNA triplex or double duplexes without denaturing the DNA duplex.
  • miRNA inhibitors of PNA-CPP: PNA binds complementary RNA more strongly than DNA or RNA does. PNA miRNA inhibitors can be conjugated to cell penetrating peptide without the need for transfection reagents for cell entry.
  • Double strand DNA invasion and capture Biotinylated-PNA: Because of its uncharged polyamide backbone, PNA can hybridize to negatively charged DNA or RNA without electrostatic repulsion.

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