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Quality and Documentation

Peptide Quality Control and Documentation

Every LifeTein peptide ships with a paper trail you can check for yourself: a certificate of analysis, the HPLC chromatogram behind the purity figure, and the mass spectrum behind the identity. This page explains what each document contains, what it does and does not prove, how long we keep samples and records, and how to raise a question if a result does not look right.

In every shipment:
  • Certificate of analysis (CoA) for the lot
  • HPLC report with the chromatogram and peak table
  • Mass spectrometry (MS) report with the observed mass

What the certificate of analysis lists

FieldWhat it tells you
Catalog or order numberLinks the vial to your order and to our internal batch record.
Peptide nameThe name used on your order.
Sequence and modificationsThe full sequence as made, including terminal groups, labels and other modifications. Check this first against what you ordered.
Quantity and vial splitAmount supplied and how it was divided between vials.
HPLC purityThe purity measured on the HPLC report for this lot.
Molecular formula and molecular weightThe values calculated for the sequence and modifications as ordered, for comparison with the MS report.
Release signature and dateShows that the lot was reviewed and released by our laboratory.
Warranty termsThe conditions of our product warranty, including the notice period for any claim.

What the HPLC report shows

A schematic chromatogram, not a real lot. Absorbance against retention time, with a large main peak for the target peptide, a small earlier peak labelled as a deletion sequence, and a small later peak labelled as an oxidation product 16 daltons heavier. Purity is the main peak area divided by the total integrated area.
How to read the trace that comes with your peptide. This is a schematic drawn for explanation, not a LifeTein lot — your report shows the real chromatogram for your own peptide.

The HPLC report shows the chromatogram of the lot and the peak table used to calculate purity. Purity is the share of the detected signal that belongs to the main peak. It tells you how much of the peptide material in the vial is the target sequence compared with closely related by-products such as deletion or truncated sequences.

HPLC purity is not the same as peptide content. A lyophilized peptide also contains counter-ions and residual water, which do not appear in the purity figure. If your work depends on knowing exactly how much peptide is in a solution, read What is net peptide content? and consider adding a content measurement to your order. For help choosing a purity level, see How to choose peptide purity.

What the mass spectrometry report confirms

The MS report confirms identity: the observed mass should match the molecular weight on the certificate, within the accuracy of the method. A correct mass confirms that the expected composition is present, including any modification that changes the mass.

Mass alone cannot tell apart peptides with exactly the same composition, such as a sequence with two residues swapped, or a peptide with an L- to D-amino acid change. Where that distinction matters for your study, ask us before ordering about additional analysis.

Reading the two reports together

The HPLC report tells you how pure the material is; the MS report tells you what the main component is. You need both. A single clean peak with the wrong mass, or the right mass with a crowded chromatogram, would each be a reason to contact us.

Our quality system

LifeTein works to an ISO-based quality manual covering document control, training, equipment, production records, release and the handling of customer feedback. Peptides are made and released for research use. LifeTein does not manufacture under GMP and makes no GMP claim for its products.

How long we keep samples and records

ItemKept for
Retained peptide sample from the lot3 months
Batch record2 years

The retained sample lets us re-test the same material if you see an unexpected result, so the sooner you contact us, the more we can check.

If something does not look right

  1. Compare the sequence and modifications on the certificate with your order.
  2. Check that the observed mass in the MS report matches the molecular weight on the certificate.
  3. Email sales@lifetein.com with your order number and a short description of the problem. Our warranty requires written notice within 30 days of receipt; see our terms and conditions.

Related pages

Frequently asked questions

What documents come with a LifeTein peptide?

Every peptide ships with a certificate of analysis, an HPLC report and a mass spectrometry report for the lot.

Does HPLC purity tell me how much peptide is in the vial?

No. HPLC purity is the share of the detected signal in the main peak. The vial also contains counter-ions and water, so net peptide content is lower than the gross weight. A separate content measurement is needed if exact amounts matter.

How long does LifeTein keep retained samples and batch records?

Retained peptide samples are kept for 3 months and batch records for 2 years.

Are LifeTein peptides made under GMP?

No. LifeTein works to an ISO-based quality manual and supplies peptides for research use only. It makes no GMP claim for its products.

Research use only. LifeTein peptides and proteins are supplied for laboratory research use. They are not for human or veterinary use, and not for diagnostic or therapeutic use. See our research use policy.