Peptide Synthesis: Handling and Storage of Synthetic Peptides

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Frequently Asked Questions (FAQ)

References using synthetic peptides and antibodies from LifeTein. Full Publication List of 2017.

Ask Me How to Handle Your Peptides!

How do I choose the best level of peptide purity for my research?

Crude peptides are not recommended for biological assays. Crude peptides may contain large amounts of non-peptide impurities such as residual solvents, scavengers from cleavage, TFA, and other truncated peptides. TFA cannot be removed. Peptides are usually delivered as TFA salt. If residual TFA is a problem for your experiment, we recommend other salt forms such as acetate and hydrochloride. These salt forms are usually 20-30% more expensive than regular TFA salt. This is due to the peptide loss that takes place during the salt conversion and the greater amounts of raw materials required.

LifeTein® recommends the following levels of peptide purity for various projects:

>75% purity

  • Peptide arrays
  • Antigens for antibody production
  • Competitive elution chromatography
  • ELISA standards for measuring antisera titers

>80% purity

  • Western blotting studies (non-quantitative)
  • Enzyme-substrate studies (non-quantitative)
  • Peptide blocking studies (non-quantitative)
  • Affinity purification
  • Phosphorylation assays
  • Protein electrophoresis applications and immunocytochemistry

>95% purity

  • ELISA standards and RIA protocols (quantitative)
  • Receptor-ligand interaction studies (quantitative)
  • In vitro bioassays and in vivo studies
  • Enzyme studies and blocking assays (quantitative)
  • NMR studies
  • Mass spectrometry
  • Other quantitative assays

>98% purity

  • SAR Studies
  • Clinical trials
  • APIs (Active Pharmaceutical Ingredients)
  • Commercial products
  • X-ray crystallography studies
  • Other sensitive experiments: enzyme-substrate studies, receptor-ligand interaction studies, blocking and competition assays