Motilin, porcine

Motilin, porcine is a synthetic research peptide in the motilin / motilin receptor family. Motilin is a 22-residue gastrointestinal peptide hormone and the endogenous ligand of the motilin receptor. Receptor mutagenesis and structural studies identify specific extracellular and transmembrane interactions that contribute to peptide recognition. Motilin variants are useful for receptor-binding, calcium-signaling, ligand-recognition, and gastrointestinal peptide structure-function studies.

• receptor or ligand-binding assays
• competition studies
• biochemical or enzymatic assays
• analytical LC-MS/HPLC methods
• antibody controls
• cell-based experiments
• comparative structure-activity studies

For a fragment, residue-substituted analog, stereochemical variant, labeled peptide, or terminally modified form, experimental behavior should be evaluated for the exact construct rather than assumed to match the native parent peptide. Sequence-dependent solubility, aggregation, adsorption, oxidation, and stability should be considered when establishing reconstitution, storage, and assay conditions.

1. Differential determinants for peptide and non-peptidyl agonist binding to the motilin receptor
2. Structural basis for motilin recognition and activation of the motilin receptor