Human Serum Albumin Protein (LTP10601)
$ 57 In stock Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier.
Recombinant Human Serum Albumin Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Asp25-Leu609.
The protein has a predicted MW of 69.4 kDa. Due to glycosylation, the protein migrates to 69-70 kDa based on the Tris-Bis PAGE result.
The affinity constant of 0.728 uM as determined in SPR assay (Biacore T200). See testing image for detail.
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipped at ambient temperature.
Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.