Human SERPINF2/A2AP Protein (LTP10439)

Product Name
Human SERPINF2/A2AP Protein (LTP10439)
Catalog Number
LTP10439
Quantity
100ug
Price
$360 In stock
Background
Large-pool solvent/detergent (SD) plasma for transfusion exhibits reduced alpha 2-antiplasmin (alpha2-AP; SERPINF2) functional activity. The reason for the loss of alpha2-AP has not been described and could be due to the SD incubation itself and/or to the processing steps implemented to remove the solvent and the detergent.
Description
Recombinant Human SERPINF2/A2AP Protein is expressed from Expi293 with His tag at the C-terminal. It contains Met28-Lys491.
Target
SERPINF2/A2AP
Species
Human
Gene/Protein ID
P08697-1
Exact Sequence
Met28-Lys491
Molecular Weight
The protein has a predicted MW of 52.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human SERPINF2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 0.27ug/ml determined by ELISA. See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human SERPINF2/A2AP Protein (LTP10439)
  • 5 Units in Stock
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