|
| Human PLAU/uPA Protein (active form) (LTP10273) |
|
| LTP10273 |
|
| 100ug |
|
|
$450 In stock |
| Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC. |
| Recombinant Human PLAU/uPA Protein (active form) is expressed from Expi293 with His tag at the C-terminal. It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-terminal fragment (Ser21-Lys155). |
|
| PLAU/uPA |
|
| Human |
|
| P00749-1 |
|
| Ser21-Leu431 |
|
| The protein has a predicted MW of 47.5 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa(long chain A), 35-38 kDa(chain B) and 52-60 kDa(long chain A&chain B) based on the Tris-Bis PAGE result. |
|
| Immobilized Human PLAU, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). The affinity constant of 5.87 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
|
| C-His |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
