Human Periostin/OSF-2 Protein (LTP10299)

Product Name
Human Periostin/OSF-2 Protein (LTP10299)
Catalog Number
LTP10299
Quantity
100ug
Price
$496 In stock
Background
Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.
Description
Recombinant Human Periostin/OSF-2 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asn22-Gln836.
Target
Periostin/OSF-2
Species
Human
Gene/Protein ID
Q15063
Exact Sequence
Asn22-Gln836
Molecular Weight
The protein has a predicted MW of 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human Periostin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human Periostin/OSF-2 Protein (LTP10299)
  • 5 Units in Stock

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