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| Human MUC18/CD146 Protein (LTP10343) |
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| LTP10343 |
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| 100ug |
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$450 In stock |
| METCAM/MUC18 is an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family. It can carry out common functions of CAMs which is to perform intercellular interactions and interaction of cell with extracellular matrix in tumor microenvironment, to interact with various signaling pathways and to regulate general behaviors of cells.The above positive correlation is fortuitous; actually METCAM/MUC18 acts as a tumor and metastasis suppressor for the ovarian carcinoma cells. |
| Recombinant Human MUC18/CD146 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Val24-Gly559. |
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| MUC18/CD146 |
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| Human |
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| P43121 |
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| Val24-Gly559 |
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| The protein has a predicted MW of 60.8 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human MUC18, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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