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| Human IL-31 Protein (LTP10520) |
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| LTP10520 |
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| 100ug |
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$473 In stock |
| Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells. |
| Recombinant Human IL-31 Protein is expressed from Expi293 with His tag at the N-terminal. It contains Ser24-Thr164. |
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| IL-31 |
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| Human |
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| Q6EBC2-1 |
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| Ser24-Thr164 |
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| The protein has a predicted MW of 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15ug/ml determined by ELISA (QC Test). The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| N-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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