Human IL-13Ra1 Protein (LTP10862)
$ 249 In stock Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Recombinant Human IL-13Ra1 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Ala27-Thr343.
The protein has a predicted MW of 63.4 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on the Tris-Bis PAGE result.
Immobilized Human IL-13, His Tag at 5ug/ml (100ul/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 4.7ug/ml determined by ELISA (QC Test). The affinity constant of 53.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipped at ambient temperature.
Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.