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| Human IFN alpha 1 Protein (LTP10182) |
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| LTP10182 |
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| 100ug |
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$473 In stock |
| IFN-?, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. |
| Recombinant Human IFN alpha 1 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Cys24-Glu189. |
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| IFN alpha 1 |
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| Human |
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| P01562 |
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| Cys24-Glu189 |
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| The protein has a predicted MW of 46.14 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on the Tris-Bis PAGE result. |
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| The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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