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| Human HGF Protein (LTP10402) |
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| LTP10402 |
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| 100ug |
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$519 In stock |
| Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types. |
| Recombinant Human HGF Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln32-Ser728. |
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| HGF |
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| Human |
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| P14210-1 |
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| Gln32-Ser728 |
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| The HGF single chain can be processed into the active form of disulfide-linked heterodimer of ? and ? chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to ? chain and ? chain based on Bis-Tris PAGE result. |
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| Immobilized Human HGF, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 52.8ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl, 200mM L-Arginine (pH 7.5). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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