Human GPRC5D Protein-VLP (LTP10647)
$ 1576 In stock Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.
Recombinant Human GPRC5D Protein-VLP is expressed from Expi293. It contains Met1-Val345.
The target protein has a predicted MW of 39.6 kDa.
Immobilized Human GPRC5D VLP at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipped at ambient temperature.
Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.