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| Human Factor H/CFH Protein (LTP10332) |
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| LTP10332 |
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| 100ug |
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$406 In stock |
| Factor H is the major soluble inhibitor of complement, where its binding to self markers (i.e., particular glycan structures) prevents complement activation and amplification on host surfaces. Not surprisingly, mutations and polymorphisms that affect recognition of self by factor H are associated with diseases of complement dysregulation, such as age-related macular degeneration and atypical haemolytic uremic syndrome. In addition, pathogens (i.e., non-self) and cancer cells (i.e., altered-self) can hijack factor H to evade the immune response. |
| Recombinant Human Factor H/CFH Protein is expressed from Expi293 with His tag at the C-terminal. It contains Ser860-Arg1231. |
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| Factor H/CFH |
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| Human |
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| P08603-1 |
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| Ser860-Arg1231 |
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| The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human Factor H, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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