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| Human EPHB2 Protein (LTP10436) |
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| LTP10436 |
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| 100ug |
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$360 In stock |
| EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2? is required for EPHB2 stabilization by hypoxia. |
| Recombinant Human EPHB2 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Val19-Leu543. |
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| EPHB2 |
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| Human |
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| P29323-1 |
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| Val19-Leu543 |
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| The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human EPHB2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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