Human CD55 Protein (LTP10274)

Product Name
Human CD55 Protein (LTP10274)
Catalog Number
LTP10274
Quantity
100ug
Price
$406 In stock
Background
Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection.
Description
Recombinant Human CD55 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asp35-Ser353.
Target
CD55
Species
Human
Gene/Protein ID
P08174-1
Exact Sequence
Asp35-Ser353
Molecular Weight
The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human CD55, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human CD55 Protein (LTP10274)
  • 5 Units in Stock

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