| Human CD55 Protein (LTP10274) |
| LTP10274 |
| 100ug |
|
$406 In stock |
| Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. |
| Recombinant Human CD55 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asp35-Ser353. |
| CD55 |
| Human |
| P08174-1 |
| Asp35-Ser353 |
| The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on the Tris-Bis PAGE result. |
| Immobilized Human CD55, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail. |
| C-His |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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