Human CD30 Ligand/TNFSF8 Protein (LTP10290)

Product Name
Human CD30 Ligand/TNFSF8 Protein (LTP10290)
Catalog Number
LTP10290
Quantity
100ug
Price
$473 In stock
Background
CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Description
Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from Expi293 with His tag at the N-terminal. It contains Gln63-Asp234.
Target
CD30 Ligand/TNFSF8
Species
Human
Gene/Protein ID
P32971
Exact Sequence
Gln63-Asp234
Molecular Weight
The protein has a predicted MW of 20.7 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. See testing image for detail.
Tag
N-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Human CD30 Ligand/TNFSF8 Protein (LTP10290)
  • 5 Units in Stock
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