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| Human BST1 Protein (LTP10437) |
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| LTP10437 |
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| 100ug |
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$450 In stock |
| BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism. |
| Recombinant Human BST1 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gly29-Ala293. |
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| BST1 |
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| Human |
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| Q10588-1 |
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| Gly29-Ala293 |
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| The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human BST1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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