Human ARTN Protein (LTP10460)
$ 450 In stock Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.
Recombinant Human ARTN Protein is expressed from Expi293 with hFc tag at the N-terminal. It contains Ala108-Gly220.
The protein has a predicted MW of 39.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on the Tris-Bis PAGE result.
Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Lyophilized from 0.22 um filtered solution in 4mM HCl (pH 2.8). Normally 8% trehalose is added as a protectant before lyophilization.
Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipped at ambient temperature.
Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl (pH2.8).