HPV-E6-N

HPV-E6-N is a synthetic research peptide in the human papillomavirus (HPV)-derived peptide family. Synthetic peptides derived from HPV E6 and E7 oncoproteins are widely used to map virus-specific T-cell responses and characterize HLA-restricted epitopes. HPV16 E7 residues 11-20 (YMLDLQPETT) are a well-studied HLA-A2-restricted CTL epitope, and anchor-modified analogs have been investigated for altered HLA affinity and immunogenicity. For longer E6/E7 fragments, the exact residue range determines which potential T-cell determinants are present.

• HPV-specific T-cell assays
• E6/E7 epitope mapping
• HLA peptide-binding experiments
• CTL induction studies
• native-versus-modified HPV epitope comparisons

For antigenic peptides, MHC/HLA restriction, species, viral strain or tumor-antigen isoform, residue numbering, sequence substitutions, and chemical modifications can materially affect peptide binding and cellular recognition. The exact construct should therefore be matched to the intended assay and the reference sequence used in the experimental system.

1. Generation of tumor-specific CTL recognizing HPV16 E7(11-20) in an HLA-A2-restricted manner
2. A single amino-acid substitution improves immunogenicity of the HPV16 E7(11-20) CTL epitope