Cys-FLAG Tag Peptide, DYKDDDDK

Cys-FLAG Tag Peptide, DYKDDDDK contains the canonical FLAG/DYKDDDDK epitope tag with terminal cysteine functionality.

FLAG / DYKDDDDK Epitope Tag

DYKDDDDK is one of the most widely used short peptide tags for recombinant-protein detection and purification. Its small size, hydrophilic character, and extensive antibody reagent availability make it useful in western blotting, immunoprecipitation, immunofluorescence, affinity capture, and protein-interaction studies.

Terminal Cysteine Functionality

The added cysteine provides a thiol group for maleimide conjugation, surface immobilization, carrier-protein coupling, fluorescent labeling, or other site-selective chemical modification.

• Anti-FLAG antibody controls
• Affinity assay development
• Surface immobilization
• Carrier conjugation
• Fluorescent probe preparation

References

Thermo Fisher Scientific Overview of Epitope Tagging. Technical reference.
Public source / publication

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Research Use Only. Not for human, diagnostic, clinical, or therapeutic use.

Cys-FLAG Tag Peptide, DYKDDDDK is a 17-residue synthetic peptide with the sequence Cys-Phe-Leu-Ala-Gly-Cys-Tyr-Ser-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Cys. The sequence contains 3 cysteine residues, providing potential thiol/disulfide chemistry when the thiol is available; contains 5 Asp and 0 Glu residues, contributing anionic character near neutral pH; contains 3 aromatic residues that can contribute to hydrophobic or aromatic interactions. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence.

• thiol-selective conjugation or immobilization studies
• LC-MS/HPLC analytical method development
• sequence-specific assay controls

Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions.