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C3 Peptide P16, also known as C3d Peptide P16, is a 16-amino-acid synthetic peptide derived from human complement C3d. The sequence KNRWEDPGKQLYNVEA contains the LYNVEA motif reported to participate in interaction with complement receptor 2 (CR2/CD21), a receptor expressed on B lymphocytes and involved in complement-linked immune regulation.
Complement C3 is a central component of the complement cascade. Proteolytic processing of C3 generates several biologically active fragments, including C3d, which can bind CR2/CD21 and modulate B-cell responses. C3d is widely studied as an immune-modulating fragment because it links innate complement activation to adaptive B-cell signaling.
Published Research Background
The original publication describing C3d Peptide P16 reported that this 16-amino-acid peptide, derived from human C3d, contains the LYNVEA sequence associated with C3d interaction with CR2. In that study, p16 induced tyrosine phosphorylation of phosphoproteins pp100 and pp105 and stimulated proliferation of human B lymphocytes in vitro.
These findings make C3 Peptide P16 a useful research reagent for studying complement receptor signaling, B-cell activation, and peptide fragments derived from the C3d region of complement C3.
Applications in Immunology Research
- Complement C3d and CR2/CD21 interaction studies
- B-cell signaling and activation research
- Tyrosine phosphorylation pathway studies
- Immune co-stimulation and complement biology
- Peptide mapping of functional C3d regions
- Design of complement-derived immune-modulating peptides
Mechanistic Features
C3d-derived sequence
This peptide is derived from human C3d, a complement fragment generated after C3 activation and processing. C3d plays an important role in immune recognition through interaction with CR2/CD21.
LYNVEA motif
The peptide contains the LYNVEA sequence reported to be involved in C3d interaction with CR2. This motif makes the peptide useful for receptor-binding and signaling studies.
B-cell signaling activity
Published studies reported that C3d Peptide P16 induced tyrosine phosphorylation of pp100 and pp105 and promoted human B lymphocyte proliferation in vitro, supporting its use as a tool for immune signaling research.
Use in Peptide and Immunological Assay Development
C3 Peptide P16 can be used as a chemically defined peptide reagent for studying complement-derived signaling motifs. It may also be useful for antibody development, receptor-binding studies, competitive assays, and functional peptide mapping of complement C3d.
The peptide can be customized with biotin, fluorescent dyes, terminal cysteine, PEG linkers, or carrier protein conjugation for specialized assay formats.
Customization Options
- Biotin-C3d Peptide P16 for binding or pull-down assays
- FITC-, FAM-, Cy3-, or Cy5-labeled C3d Peptide P16 for detection studies
- Cys-C3d Peptide P16 for thiol-based conjugation
- KLH, BSA, or OVA conjugation for antibody production
- PEGylated or linker-modified versions for surface immobilization
Publication
Frade R. A 16 amino acid synthetic peptide derived from human C3d regulates in vitro and in vivo tyrosine phosphorylations of the phosphoproteins pp100 and pp105 and in vitro proliferation of human B lymphocytes. 1992. PMID: 1445326.
Related LifeTein Services
Custom Peptide Synthesis
Fluorescent Peptide Labeling
Peptide Carrier Protein Conjugation
Peptide Click Chemistry and Conjugation
Research Use Only. Not for human or therapeutic use.
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