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| Biotinylated Human Siglec-10 (R119A) Protein (LTP10213) |
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| LTP10213 |
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| 100ug |
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$789 In stock |
| Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. |
| Recombinant Biotinylated Human Siglec-10(R119A) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Met17-Thr546. |
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| Siglec-10 (R119A) |
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| Human |
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| Q96LC7-1 |
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| Met17-Thr546 |
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| The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-Siglec-10 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) , His Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in 20mM Tirs 150mM NaCl, 200mM L-Arginine (pH 8.0). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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