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| Biotinylated Human Siglec-2/CD22 Protein (LTP10936) |
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| LTP10936 |
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| 100ug |
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$789 In stock |
| CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice. |
| Recombinant Biotinylated Human Siglec-2/CD22 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Asp20-Arg687. |
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| Siglec-2/CD22 |
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| Human |
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| P20273-1 |
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| Asp20-Arg687 |
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| The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-Siglec-2 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-2, His Tag with the EC50 of 19.1ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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