Biotinylated Human IL-5 R alpha/CD125 Protein (LTP10236)

Product Name
Biotinylated Human IL-5 R alpha/CD125 Protein (LTP10236)
Catalog Number
LTP10236
Quantity
100ug
Price
$789 In stock
Background
Interleukin?5 Receptor alpha (IL?5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.
Description
Recombinant Biotinylated Human IL-5 R alpha/CD125 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Asp21-Glu335.
Target
IL-5 R alpha/CD125
Species
Human
Gene/Protein ID
Q01344-1
Exact Sequence
Asp21-Glu335
Molecular Weight
The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human IL-5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human IL-5 R alpha/CD125 Protein (LTP10236)
  • 5 Units in Stock

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