Biotin-[Tyr0]-Orexin B, mouse,rat

Biotin-[Tyr0]-Orexin B, mouse, rat is a synthetic research peptide in the orexin / hypocretin family. Orexin-A and orexin-B (hypocretins) are neuropeptides that activate OX1 and OX2 receptors. Structure-activity studies using truncations and substitutions have identified sequence features that influence receptor potency and subtype preference. Defined orexin fragments and analogs are useful for receptor activation, selectivity, stability, and structure-function experiments.

• receptor-binding
• competition assays
• functional signaling assays
• enzymatic-processing or stability studies
• antibody controls
• LC-MS/HPLC method development
• comparative structure-activity experiments

For a fragment, residue-substituted analog, stereochemical variant, labeled peptide, or terminally modified form, experimental behavior should be evaluated for the exact construct rather than assumed to match the native parent peptide. Sequence-dependent solubility, aggregation, adsorption, and stability should be considered when selecting reconstitution and assay conditions.

1. Structure-activity studies of orexin A and orexin B at human orexin receptors
2. Structure-activity analysis of truncated orexin-A analogues at the orexin-1 receptor