Product Name | Anti-HEL (Hen Egg Lysozyme) mouse IgG2a agly isotype control |
Catalog Number | LT12038 |
Name | Anti-HEL (Hen Egg Lysozyme) mouse IgG2a aglycosylated form (kappa) isotype control (whole molecule), recombinant, purified, low endotoxin |
Abbreviated name | Mouse IgG2a agly |
Ig subclass | Mouse IgG2a agly, Kappa |
Host species | Mouse |
Origin | CHO-S cell |
Application | The anti-HEL (Hen Egg Lysozyme) IgG isotype control antibodies were expressed in CHO-S cells and purified with protein A affinity column. The isotype control antibodies can be used as negative controls to differentiate non-specific background signals from your target antibody signals. Whole molecule immunoglobulins may also be used as standard protein or coating protein in ELISA, Western-Blot, immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and flow cytometry (FACS). This product is also suitable for various in vitro and in vivo studies that require low endotoxin levels. LifeTein's isotype control proteins were produced under stringent endotoxin control conditions of <0.5 EU/μg. |
Quality Control | Endotoxin level: <0.5 EU/mg by the LAL method
Purity: >95% by HPLC and SDS-PAGE |
Purification Method | Protein A affinity column |
Concentration | 1 mg, Lyophilized in 20 mM PBS, pH7.4 |
Conjugation | Unconjugated |
Storage | Store at -80°C in PBS for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1mg/ml. Aliquot to avoid repeated freeze-thaw cycles. |
Product Description | The anti-HEL (Hen Egg Lysozyme) mouse IgG2a aglycosylated form (kappa) isotype control antibody was expressed in CHO-S cells and highly purified with protein A affinity column |
Scientific Background | Anti-HEL (Hen Egg Lysozyme) mouse IgG2a agly isotype control is a 4-residue synthetic peptide with the sequence Ala-Asn-Thr-Ile. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence. |
Research Applications | - LC-MS/HPLC analytical method development
- sequence-specific assay controls
|
Experimental Notes | Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions. |
