Product Name | Anti-HEL (Hen Egg Lysozyme) mouse IgG1 isotype control |
Catalog Number | LT12035 |
Name | Anti-HEL (Hen Egg Lysozyme) mouse IgG1 isotype control (whole molecule), recombinant, purified, low endotoxin |
Abbreviated name | Mouse IgG1 |
Ig subclass | Mouse IgG1, Kappa |
Host species | Mouse |
Origin | CHO-S cell |
Application | The anti HEL (Hen Egg Lysozyme) IgG isotype control antibodies were expressed in CHO-S cells and purified with protein A affinity column. The isotype control antibodies can be used as negative controls to differentiate non-specific background signal from your target antibody signals. Whole molecule immunoglobulins may also be used as standard protein or coating protein in ELISA, Western-Blot, immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and flow cytometry (FACS). This product is also suitable for various in vitro and in vivo studies which require low endotoxin level. LifeTein's isotype control proteins were produced under stringent endotoxin control conditions of <0.5 EU/μg. |
Quality Control | Endotoxin level: <0.5 EU/mg by the LAL method
Purity: >95% by HPLC and SDS-PAGE |
Purification Method | Protein A affinity column |
Concentration | 1 mg, Lyophilized in 20 mM PBS, pH7.4 |
Conjugation | Unconjugated |
Storage | Store at -80°C in PBS for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1mg/ml. Aliquot to avoid repeated freeze-thaw cycles. |
Scientific Background | Anti-HEL (Hen Egg Lysozyme) mouse IgG1 isotype control is a 4-residue synthetic peptide with the sequence Ala-Asn-Thr-Ile. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence. |
Research Applications | - LC-MS/HPLC analytical method development
- sequence-specific assay controls
|
Experimental Notes | Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions. |
