FITC Peptide Labeling
FITC is one of the most widely used fluorescent labels for peptides. It is commonly used in fluorescence microscopy, flow cytometry, uptake studies, and general biomolecular tracking applications.
Key Properties
| Excitation / Emission | 495 / 519 nm |
| Color | Green |
| Laser Compatibility | 488 nm |
| Typical Use | Microscopy, flow cytometry, uptake studies |
| Notes | Commonly used with Ahx spacer for peptide labeling |
FITC Spectrum
Why Use FITC?
- Widely recognized green fluorescent label
- Compatible with common 488 nm excitation sources
- Useful for peptide tracking and cellular assays
- Well established in many antibody and peptide workflows
Peptide Labeling Options
- N-terminal FITC labeling
- Lysine side-chain labeling
- Cysteine-directed conjugation in selected designs
- Optional Ahx or β-Ala spacer to reduce steric interference
Tip: FITC-labeled peptides often benefit from an Ahx spacer to improve accessibility and reduce steric hindrance.
Related Dyes
