Rabbit Monoclonal Antibody Service – SIMBA^TM

Rabbit Monoclonal Antibody Service – SIMBA^TM (Rabbit🐇, Mouse🐇, or Llama🦙)

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LifeTein provides rabbit monoclonal antibody services through the SIngle Monoclonal B cell Antibody (SIMBA^TM) workflow, which is based on antigen-specific single B cell screening, recovery of antibody variable regions, recombinant expression, and clone validation. This service is especially relevant for difficult epitopes, modified targets, peptide antigens, and projects where rabbit monoclonal antibodies may offer stronger performance than standard mouse monoclonal approaches.

The service positioning here is intended to reflect current single-B-cell rabbit monoclonal workflows used in the industry, including immunization, antigen-specific B-cell selection, VH/VL recovery, recombinant expression, and screening.

SIMBA^TM Workflow Overview

Main concept	Antigen-specific single B cell screening followed by VH/VL recovery and recombinant antibody expression
Main immunogens	Peptides, conjugated peptides, haptens, recombinant proteins, and selected specialized immunogens
Main output	Validated recombinant monoclonal antibody candidates plus sequence information for selected clones
Typical strength	Useful for difficult epitopes, PTM sites, small molecules, rare epitopes, and peptide-driven targets

Why Rabbit Monoclonal Antibodies?

Rabbit antibodies often show strong affinity to difficult or less immunogenic epitopes
They are frequently chosen for PTM sites, small molecules, haptens, and rare epitopes
They can be a good fit when standard mouse workflows are not ideal for the target

Typical SIMBA^TM Workflow

Immunization using peptide, conjugated peptide, hapten, or recombinant protein immunogen
Selection of the best responding animal based on screening data
Isolation of antigen-specific B cells
Recovery of antibody variable-region genes from selected cells
Recombinant expression and screening of antibody candidates
Selection of positive clones and delivery of purified antibody candidates with sequence information

Typical Project Types

Peptide epitope targets
Phospho-specific and PTM-specific targets
Hapten or small-molecule-related projects
Rare or difficult epitopes
Targets requiring recombinant rabbit mAb output rather than a hybridoma-style mouse workflow

Example Timeline Structure

Phase	Service	Description	Typical Timeline
Phase I	Immunization and primary screening	Immunization, bleed monitoring, ELISA or related screening, and selection of the strongest responding animal.	~6–9 weeks
Phase IIa	Single B cell screening	Antigen-specific B cell selection and primary screening of candidate populations.	~2 weeks
Phase IIb	VH/VL recovery and construct generation	Recovery of antibody genes from selected positive cells and preparation for recombinant expression.	~2 weeks
Phase III	Recombinant expression and validation	Expression of selected rabbit mAb candidates, validation screening, and selection of final clones for delivery.	~3 weeks

Actual timelines and deliverables depend on antigen behavior, screen performance, and final clone selection.

Best fit for SIMBA^TM

This service is best positioned for researchers who want recombinant rabbit monoclonal candidates from a single-B-cell style workflow, especially for difficult peptide targets, phospho-sites, modified epitopes, or small-molecule-related immunogens.

Related Antibody Services

Reference:

References using synthetic peptides and antibodies from LifeTein. See search results page from the Google Scholar.

Herklotz, V., Zhang, M., Nascimento, T. et al. Bimodal centromeres in pentaploid dogroses shed light on their unique meiosis. Nature (2025). https://doi.org/10.1038/s41586-025-09171-z

The polyclonal antibodies recognizing CENH3 protein and kinetochore protein KNL1 were generated by LifeTein.

Greene SE, Huang Y, Fischer K, Rosa BA, Martin J, Mitreva M, et al. (2024) A novel antigen biomarker for detection of high-level of Loa loa microfilaremia. PLoS Negl Trop Dis 18(9): e0012461. https://doi.org/10.1371/journal.pntd.0012461

Polyclonal rabbit antibodies to Ll-Bhp-1 were commercially produced using recombinant Ll-Bhp-1 and purified by protein A affinity chromatography (LifeTein, Somerset, NJ, USA).

Padula AM. Immunoreactivity of eastern small eyed snake (Cryptophis nigrescens) venom towards species-specific antibodies of five medically important venomous Australian elapids. Aust Vet J. 2024 Jul 15. doi: 10.1111/avj.13356. Epub ahead of print. PMID: 39009475.

LifeTein produced a series of snake venom antibodies. Venom species used for rabbit IgG production: Tiger, Brown, Black, Death adder, and Taipan. The snake venoms were used for immunization of rabbits to generate polyclonal antibodies.

Protein A purified rabbit IgG specific for each venom species (Table 1) were purchased from a commercial supplier (Lifetein, Somerset, NJ, USA).

Holocentromeres can consist of merely a few megabase-sized satellite arrays Nature CommunicationsNat Commun 14, 3502 (2023). https://doi.org/10.1038/s41467-023-38922-7

LifeTein designed and synthesized a series of peptides: CENH3, MIS12 and NDC80. The peptides were used for immunization of rabbits to generate polyclonal antibodies.

... The peptide synthesis, immunization, and antibody purification were performed by LifeTein...

Dias, Y., Mata-Sucre, Y., Thangavel, G., Costa, L., Báez, M., Houben, A., Marques, A., & Pedrosa-Harand, A. (2024). How diverse a monocentric chromosome can be? Repeatome and centromeric organization of Juncus effusus (Juncaceae). The Plant Journal, 118(6), 1832-1847. https://doi.org/10.1111/tpj.16712

...antibodies in rabbits using the service of Lifetein, LLC (Somerset, NJ...

Jia Shen. et al. EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Nature 497, 383–387 (16 May 2013), doi:10.1038/nature12080

LifeTein helped designed and synthesized a series of phosphorylated peptides. Then the peptides were used for phospho-specific antibody productions. The phospo-specific antibodies by LifeTein were confirmed to react with the epidermal growth factor receptor (EGFR). The Hung's lab showed that AGO2-Y393 phosphorylation mediates EGFR-enhanced cell survival and invasiveness under hypoxia. These findings suggest that modulation of miRNA biogenesis is important for stress response in tumour cells.

... The following peptides were chemically synthesized for antibody production in mice (Lifetein), Elisa verification (Lifetein) and peptide competition assay in immunohistochemistry (IHC)... Supplementary information