[Tyr1]-pTH (1-34) (rat)

[Tyr1]-pTH (1-34) (rat) is a synthetic research peptide in the parathyroid hormone / PTH-related peptide family. Parathyroid hormone (PTH) and PTH-related peptide (PTHrP) signal through PTH1R, a class B GPCR. Structural and structure-activity studies support a two-part interaction model in which the C-terminal portion of PTH(1-34) contributes strongly to receptor binding while the N-terminal portion is important for receptor activation. PTH/PTHrP fragments, substitutions, and terminally modified analogs are therefore useful for dissecting binding, activation, and peptide conformation.

• receptor or ligand-binding assays
• competition studies
• biochemical or enzymatic assays
• analytical LC-MS/HPLC methods
• antibody controls
• cell-based experiments
• comparative structure-activity studies

For a fragment, residue-substituted analog, stereochemical variant, labeled peptide, or terminally modified form, experimental behavior should be evaluated for the exact construct rather than assumed to match the native parent peptide. Sequence-dependent solubility, aggregation, adsorption, oxidation, and stability should be considered when establishing reconstitution, storage, and assay conditions.

1. Crystal structure of human parathyroid hormone 1-34 at 0.9-A resolution
2. Molecular recognition of parathyroid hormone by its G protein-coupled receptor