TRH (free acid)

TRH (free acid) is a synthetic research peptide in the thyrotropin-releasing hormone (TRH) family. Thyrotropin-releasing hormone is the tripeptide pyroglutamyl-histidyl-prolinamide and acts through TRH receptors. Analogs that modify the N terminus, central histidine, C-terminal amide, or peptide conformation have been used to examine receptor recognition, metabolic stability, endocrine activity, and central nervous system actions.

• receptor or ligand-binding assays
• competition studies
• biochemical or enzymatic assays
• analytical LC-MS/HPLC methods
• antibody controls
• cell-based experiments
• comparative structure-activity studies

For a fragment, residue-substituted analog, stereochemical variant, labeled peptide, or terminally modified form, experimental behavior should be evaluated for the exact construct rather than assumed to match the native parent peptide. Sequence-dependent solubility, aggregation, adsorption, oxidation, and stability should be considered when establishing reconstitution, storage, and assay conditions.

1. Structure-activity relationships of TRH analogs in rat spinal cord injury
2. Restricted analogues provide evidence of a biologically active conformation of TRH