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Sulfo-Cy7.5-GPLGVRGC MMP-Responsive Peptide uses the published matrix-metalloproteinase-responsive peptide GPLGVRGC. The related GPLGVR motif is a widely used substrate sequence for MMP-responsive imaging, drug-delivery, and biomaterial systems.
MMP-Responsive Peptide Substrate
Matrix metalloproteinases are zinc-dependent extracellular proteases that remodel extracellular matrix proteins. Increased MMP activity is studied in cancer, inflammation, tissue remodeling, and wound-healing systems. Short MMP-cleavable peptides provide convenient substrates for constructing activatable probes and enzyme-responsive linkers.
Published work directly tested GPLGVRGC cleavage with activated human MMP-2 and used the peptide to construct PEG-peptide conjugates and activatable nanoparticle systems. The cysteine at the C-terminus provides a convenient thiol for conjugation while the GPLGVR region contains the protease-sensitive element.
Sulfo-Cy7.5 Fluorescent Reporter
LT8679 includes an N-terminal Sulfo-Cy7.5 label and C-terminal amidation. Sulfonated near-infrared dyes are useful in optical imaging research because their long-wavelength excitation/emission can reduce interference from many visible-range cellular fluorophores.
Research Applications
- MMP-2/MMP-9 substrate assays
- Protease-activated imaging probes
- Enzyme-responsive nanoparticle systems
- Hydrogel and biomaterial cleavage studies
- Protease kinetics
- Near-infrared fluorescence studies
References
Lee H, et al. Polydopamine-based surface modification for the development of peritumorally activatable nanoparticles. Pharm Res. 2013.
Public source / publication
Chuang CH, et al. In vivo positron emission tomography imaging of protease activity by generation of a hydrophobic product from a noninhibitory protease substrate. Clin Cancer Res. 2012.
Public source / publication Related LifeTein Services
Custom Peptide Synthesis
Fluorescent Peptide Labeling
Peptide Click Chemistry and Conjugation
Peptide Carrier Protein Conjugation
Research Use Only. Not for human, diagnostic, clinical, or therapeutic use.
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