Scientific Background | ST{pT}PGGDYKDDDDK is a synthetic FLAG epitope-tag-containing peptide. This product contains the FLAG epitope sequence DYKDDDDK. FLAG is a compact acidic epitope tag widely used for antibody-based detection, immunoaffinity capture, purification, and analytical characterization of tagged proteins. Synthetic FLAG peptide can also serve as a competition/elution reagent and a defined anti-FLAG binding control. |
Experimental Notes | The behavior of a motif embedded in a longer peptide can differ from the isolated tag or parent sequence. Flanking residues, linkers, phosphorylation, fluorophores, affinity handles, cyclization, and terminal modifications may alter accessibility, binding, uptake, or assay performance. |
