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The peptide sequence CKENALLRYLLDKDD corresponds to a segment derived from the human steroid receptor coactivator-2 (SRC2), also known as transcriptional intermediary factor 2 (TIF2). This 15-amino-acid peptide is utilized in molecular biology research to study interactions with nuclear receptors, particularly the androgen receptor (AR).
Role in Nuclear Receptor Interaction Studies
The CKENALLRYLLDKDD peptide is employed to investigate the binding dynamics between coactivator proteins and nuclear receptors. By mimicking a portion of the SRC2 protein, this peptide aids in elucidating the mechanisms of transcriptional regulation mediated by nuclear receptors. Such studies are crucial for understanding gene expression control and have implications in fields like endocrinology and oncology.
Applications in Research:
In experimental settings, the CKENALLRYLLDKDD peptide can be labeled with fluorescent tags to facilitate the detection and quantification of binding events. This approach allows researchers to assess the affinity and specificity of interactions between coactivators and nuclear receptors, contributing to the development of therapeutic agents targeting these pathways.
The CKENALLRYLLDKDD peptide serves as a valuable tool in the study of nuclear receptor-coactivator interactions. Its application enhances our understanding of transcriptional regulation mechanisms and supports the advancement of targeted therapies in hormone-related diseases. | 