Protein Kinase G Substrate; G-Subtide

Protein Kinase G Substrate; G-Subtide is a synthetic research peptide in the protein kinase G peptide substrate family. cGMP-dependent protein kinase G phosphorylates defined Ser/Thr-containing peptide motifs and is routinely studied with synthetic peptide substrates such as G-subtide-type sequences. These reagents support quantitative kinase assays, substrate-recognition studies, and comparison with related cyclic-nucleotide-dependent kinases.

• PKG activity assays
• cGMP-dependent phosphorylation studies
• kinase substrate comparisons
• phosphorylation kinetics

The exact sequence, reporter group, phosphorylation state, stereochemistry, and terminal modifications should be considered when comparing this reagent with the parent peptide or published assay substrates. Enzyme concentration, buffer composition, cofactors, pH, ionic strength, and substrate concentration can also affect measured activity.

1. Synthetic peptide substrates for cyclic-nucleotide-dependent protein kinase studies