Protein Kinase A Substrate

Protein Kinase A Substrate is a synthetic research peptide in the protein kinase A peptide substrate family. Synthetic peptide substrates are established tools for measuring cAMP-dependent protein kinase A activity. Kemptide (LRRASLG) is a canonical PKA substrate used for kinetic, structural, and assay-development studies. Related peptide substrates and labeled variants permit quantitative measurement of phosphorylation and substrate-recognition requirements.

• PKA activity assays
• phosphorylation kinetics
• kinase substrate-specificity studies
• protein kinase inhibitor testing

The exact sequence, reporter group, phosphorylation state, stereochemistry, and terminal modifications should be considered when comparing this reagent with the parent peptide or published assay substrates. Enzyme concentration, buffer composition, cofactors, pH, ionic strength, and substrate concentration can also affect measured activity.

1. Studies on the kinetic mechanism of cAMP-dependent protein kinase using Kemptide
2. A microchip-based enzyme assay for protein kinase A using Kemptide