PARP1 Y158 Peptide

Our Phosphorylated PARP1 Y158 Peptide, with the sequence C-EKPQLGMIDRW-pY-HPG-S-FVKNREE, is a highly specialized research tool designed for advanced studies in cancer biology and therapeutic development. This peptide is a crucial component in understanding the role of PARP1 phosphorylation in DNA repair mechanisms and the development of resistance to PARP inhibitors in cancer treatment.

Key Features:

1. Specific Phosphorylation Site: The peptide includes a phosphorylation at the Y158 amino acid, a critical site for the study of PARP1's role in DNA damage response and repair.

2. Relevance in Cancer Research: Y158 phosphorylation of PARP1 by FGFR3 has been shown to contribute to the resolution of PARP trapping in tumor cells, a key mechanism in cancer progression and treatment resistance.

3. Utility in Drug Resistance Studies: The peptide aids in understanding how FGFR-mediated phosphorylation of PARP1 affects the efficacy of PARP inhibitors, providing insights into overcoming drug resistance.

4. Biomarker Potential: Phosphorylated Y158 PARP1 serves as a potential biomarker for stratifying patients for combination treatments with FGFR inhibitors and PARP inhibitors.

5. Structural Insights: The location of Y158 near the PARP1 ZF2 domain and DNA-interacting residues suggests its involvement in protein structure stabilization and PARP1-DNA interaction dynamics.

Applications:

• Targeted Cancer Therapy Research: Essential for developing combination therapies using FGFR inhibitors and PARP inhibitors.
• Molecular Biology Studies: Useful in exploring the structural and functional aspects of PARP1 in DNA repair.
• Biomarker Development: Aids in identifying patients who would benefit from specific cancer treatments based on PARP1 phosphorylation status.

Quality Assurance:

Our Phosphorylated PARP1 Y158 Peptide is synthesized with precision, ensuring high purity and consistency. Each batch undergoes rigorous quality control and is accompanied by a comprehensive Certificate of Analysis.

PARP1 Y158 Peptide is a 11-residue synthetic peptide with the sequence Glu-Lys-Pro-Gln-Leu-Gly-Met-Ile-Asp-Arg-Trp. phosphotyrosine defines a tyrosine-phosphorylation state. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence.

• phospho-specific antibody controls
• phosphoproteomics or kinase/phosphatase assay development
• LC-MS/HPLC analytical method development
• sequence-specific assay controls

Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions.