p60c-src Substrate I

p60c-src Substrate I is a synthetic Src-family tyrosine-kinase substrate/regulatory peptide. Src-family kinases recognize sequence context surrounding tyrosine phosphoacceptors, while phosphorylation of regulatory Src tyrosines can alter kinase conformation and activity. Synthetic Src substrate, autophosphorylation-site, and C-terminal regulatory peptides are useful for tyrosine-kinase assays and phosphopeptide-binding studies. Sequence context should be considered because tyrosine kinases show distinct intrinsic substrate preferences.

• Src-family kinase activity assays
• tyrosine phosphorylation studies
• Src regulatory-site research
• phosphopeptide binding and analytical controls

The exact sequence, phosphorylation state, residue numbering, terminal modifications, labels, and species context should be matched to the intended assay. Sequence motifs support experimental interpretation but do not by themselves establish absolute enzyme specificity or native biological activity.

1. Use of synthetic peptide libraries to probe protein kinase substrate specificity
2. Intrinsic substrate specificity of the human tyrosine kinome