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| Mouse SPP1/OPN Protein (LTP10487) |
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| LTP10487 |
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| 100ug |
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$563 In stock |
| Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells. |
| Recombinant Mouse SPP1/OPN Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu17-Asn294. |
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| SPP1/OPN |
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| Mouse |
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| P10923 |
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| Leu17-Asn294 |
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| The protein has a predicted MW of 31.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on the Tris-Bis PAGE result. |
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| 0 |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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