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| Mouse FOLR4/Juno Protein (LTP10300) |
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| LTP10300 |
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| 100ug |
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$450 In stock |
| Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor. |
| Recombinant Mouse FOLR4/Juno Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gly20-Gly222. |
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| FOLR4/Juno |
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| Mouse |
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| Q9EQF4-1 |
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| Gly20-Gly222 |
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| The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on the Tris-Bis PAGE result. |
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| 0 |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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