Mouse FOLR4/Juno Protein (LTP10300)

Product Name
Mouse FOLR4/Juno Protein (LTP10300)
Catalog Number
LTP10300
Quantity
100ug
Price
$450 In stock
Background
Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor.
Description
Recombinant Mouse FOLR4/Juno Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gly20-Gly222.
Target
FOLR4/Juno
Species
Mouse
Gene/Protein ID
Q9EQF4-1
Exact Sequence
Gly20-Gly222
Molecular Weight
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on the Tris-Bis PAGE result.
Activity
0
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

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Mouse FOLR4/Juno Protein (LTP10300)
  • 5 Units in Stock
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