[Leu144,Arg147]-Myelin Proteolipid Protein(139-151)

This entry is listed as the [Leu144, Arg147] analogue of myelin proteolipid protein 139-151, the altered peptide ligand best known for acting as a T-cell receptor antagonist and for inducing regulatory T cells that mediate bystander suppression in experimental autoimmune encephalomyelitis.

The two substitutions sit at the dominant and secondary receptor contacts of the epitope respectively, and the antagonist phenotype is associated with the combination rather than with either alone. Because the analogue blocks activation rather than driving it, and because the regulatory population it elicits acts on responses to unrelated myelin antigens, it has been used as a reference compound in antigen-specific immunotherapy work rather than purely as a negative control.

Please note a discrepancy in the listed sequence. The sequence recorded for this entry begins Ser-His, whereas the native epitope and the corresponding entry LT0627 both begin His-Ser. The stated molecular weights are identical, which is consistent with a transposition of the first two residues in the record rather than a different peptide. We recommend confirming the intended sequence against the certificate of analysis before ordering, and we are reviewing this entry.

• T-cell receptor antagonism and altered peptide ligand studies
• Bystander suppression and regulatory T-cell induction
• EAE prevention and antigen-specific immunotherapy models
• MHC class II binding and competition assays

1. A T cell receptor antagonist peptide induces T cells that mediate bystander suppression and prevent autoimmune encephalomyelitis induced with multiple myelin antigens. PNAS (1997) 94:9279. doi:10.1073/pnas.94.17.9279

2. Immune responses of linear and cyclic PLP 139-151 mutant peptides in SJL/J mice. Linear and cyclic PLP mutants

3. Induction of active and adoptive relapsing experimental autoimmune encephalomyelitis (EAE) using an encephalitogenic epitope of proteolipid protein. (1992). PMID 1376328

4. Fine specificity of CD4+ T cell responses to the dominant encephalitogenic PLP 139-151 peptide in SJL/J mice. (1994). PMID 7528357