KRWIILGLNK

Peptide KRWIILGLNK is a well-established epitope derived from the HIV-1 Gag protein (p24) and recognized in the context of HLA-B*2705 for CD8⁺ T-cell responses.

Peptide Sequence: KRWIILGLNK (H-KRWIILGLNK-OH)

Source: HIV-1 Gag polyprotein (p24 region, residues 263–272)

HLA Restriction: HLA-B*2705 (MHC class I)

Applications: Used in T-cell stimulation assays (e.g., ELISPOT, ICS, cytotoxicity, proliferation) to assess CD8⁺ T-cell immune responses against HIV.

Features & Benefits:

• Immunogenic Epitope Analogue:
  KRWIILGLNF analog functions as an HLA-B*2705–restricted CD8⁺ T-cell epitope, analogous to the established KRWIILGLNK peptide.
• Functional Use in Immunoassays:
  Effective for stimulating antigen-specific CD8⁺ T cells in various assays—such as ELISPOT, intracellular cytokine staining, cytotoxicity assays, or proliferation tests.
• T-Cell Activation Studies:
  Can be utilized to challenge and characterize HLA-B*2705–restricted T-cell populations in PBMCs or splenocytes.
• Variant Exploration:
  Useful for studying the immunological impact of substituting lysine (“K”) with phenylalanine (“F”)—potentially affecting peptide–MHC binding or TCR recognition.
• Vaccine & Immunotherapy Research:
  May serve as a comparative peptide in vaccine design, immunogenicity screening, or T-cell receptor (TCR) fine-mapping efforts.

KRWIILGLNK is a 10-residue synthetic peptide with the sequence Lys-Arg-Trp-Ile-Ile-Leu-Gly-Leu-Asn-Lys. The sequence contains 2 Lys and 1 Arg residues, contributing cationic character; has a relatively high hydrophobic-residue fraction that can influence aqueous solubility and surface adsorption. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence.

• LC-MS/HPLC analytical method development
• sequence-specific assay controls

Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions.