Kemptide

Kemptide is a synthetic peptide substrate corresponding to part of the phosphorylation site sequence in porcine liver pyruvate kinase. It is phosphorylated in vitro and in vivo by cAMP-dependent protein kinase and in vitro by protein kinase C.

Kemptide is a synthetic research peptide in the Kemptide protein kinase A substrate family. Kemptide (LRRASLG) is a canonical synthetic substrate for cAMP-dependent protein kinase A. Variants carrying biotin, phosphoserine, substitutions, or linkers are useful for phosphorylation kinetics, substrate-recognition studies, affinity capture, and assay-development comparisons.

• PKA activity assays
• phosphorylation kinetics
• substrate-specificity studies
• biotinylated kinase assay development

The exact sequence, residue range, species, stereochemistry, terminal state, labels, and other modifications can materially affect activity. Match the supplied construct to the intended assay and published reference context.

1. Kinetic mechanism of cAMP-dependent protein kinase using Kemptide
2. Microchip PKA assay using Kemptide