Human/Cynomolgus/Rhesus macaque ROR1 Protein (LTP10890)
$ 203 In stock ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1), is a member of the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members.
Recombinant Human/Cynomolgus/Rhesus macaque ROR1 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Gln30-Glu403.
The protein has a predicted MW of 68.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on the Tris-Bis PAGE result.
Immobilized Human/Cynomolgus/Rhesus macaque ROR1, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ROR1 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA. See testing image for detail.
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Less than 1EU per ug by the LAL method.
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipped at ambient temperature.
Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.