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| Human TREM2 Protein (LTP10428) |
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| LTP10428 |
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| 100ug |
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$305 In stock |
| TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia. |
| Recombinant Human TREM2 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains His19-Ser174. |
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| TREM2 |
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| Human |
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| Q9NZC2-1 |
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| His19-Ser174 |
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| The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human TREM2, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 25ng/ml determined by ELISA (QC Test). The affinity constant of 68.42 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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