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| Human TNFR2/CD120b/TNFRSF1B Protein (LTP10822) |
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| LTP10822 |
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| 100ug |
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$360 In stock |
| Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. |
| Recombinant Human TNFR2/CD120b/TNFRSF1B Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu23-Asp257. |
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| TNFR2/CD120b/TNFRSF1B |
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| Human |
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| P20333-1 |
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| Leu23-Asp257 |
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| The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human TNFR2, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.6ng/ml determined by ELISA. See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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