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| Human TIGIT Protein (LTP10723) |
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| LTP10723 |
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| 100ug |
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$450 In stock |
| TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells. |
| Recombinant Human TIGIT Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Met22-Pro141. |
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| TIGIT |
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| Human |
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| Q495A1-1 |
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| Met22-Pro141 |
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| The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CD155, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 73.0ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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