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| Human SIRP alpha/CD172a Protein (LTP10746) |
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| LTP10746 |
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| 100ug |
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$406 In stock |
| Signal regulatory protein ? (SIRP?) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRP? acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP ? and inhibit macrophage-mediated destruction. |
| Recombinant Human SIRP alpha/CD172a Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Glu31-Arg370. |
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| SIRP alpha/CD172a |
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| Human |
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| P78324-1 |
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| Glu31-Arg370 |
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| The protein has a predicted MW of 64.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA (QC Test). The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-hFc |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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